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SPEX SamplePrep - Knowledge Base - Application Notes
Application Notes SP005: DNA/RNA Extraction. Apparatus: 6875 Freezer/Mill®. Application: PCR. The object of the study was to determine if there is a connection between the seriousness of arthritic diseases, especially of the knee joint and certain genes. To this end the expression profile of various genes in the tissue of healthy and diseased ...
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iRNA-seq: computational method for genome-wide assessment of acute ...
Effect of acute TNF treatment on transcription in human SGBS adipocytes as assessed by RNA-seq and RNAPII ChIP-seq. Following 10 days in vitro differentiation, human SGBS adipocytes were treated with vehicle or TNF for 90 min before harvest of RNA for total RNA-seq and chromatin for RNAPII ChIP-seq. The screenshot from the UCSC genome browser illustrates intron/exon coverage and association ...
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Hypothesis
The other half or remaining brain sections can be snap freeze in liquid nitrogen or processed immediately for RNA or protein extraction.STOP POINT: If samples cannot be immediately processed, we recommend homogenizing the tissue immediately using a Dounce homogenizer (or other methods, such as bead mill) in the appropriate buffer, making sure ...
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Professor Neena Mitter - UQ Researchers
A comprehensive high-quality DNA and RNA extraction protocol for a range of cultivars and tissue types of the woody crop avocado. Plants, 11 (3) 242. doi: 10.3390/plants11030242. miRNA communication on another level. Brosnan, Christopher A. and Mitter, Neena (2021). miRNA communication on another level. Nature Plants, 7 (10), 1328-1329. doi: 10 ...
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Gene Expression Signature in Advanced Colorectal Cancer Patients Select ...
After each extraction, a small fraction of the total RNA preparation was taken to determine the quality of the sample and the yield of total RNA. Controls analyses were performed by UV spectroscopy and analysis of total RNA profile using the Agilent RNA 6000 Nano LabChip Kit with the Agilent 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA ...
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Estrogen receptor profiles across tissues from male and Rattus ...
RNA extraction. RNA extraction was accomplished using the RNeasy MiniKit (QIAGEN, # 74106) according to the manufacturer's protocol. Purity and concentration of RNA was determined by a NanoDrop™ 2000 Spectrophotometer (Thermo Fisher Scientific) using a previously described method . Samples with a 260/280 ratio greater than 1.8 were used ...
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MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis
27 September 2019. Article history. Received: 20 May 2019. ... as compared with the absolute number of sRNA spike-in molecules (log 10) added during RNA isolation for a sRNA-seq library generated from 5 ng of total ... snap-frozen in liquid nitrogen, homogenized using a Mixer Mill MM 400 (Retsch) for 30 s with maximum amplitude, and resuspended ...
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30825 PDFs | Review articles in RNA EXTRACTION - ResearchGate
Nucleic acid extraction from biological samples is an important step for hepatitis C virus (HCV) diagnosis. However, such extractions are mostly based on silica-based column methodologies, which...
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Small RNA profiling for identification of microRNAs involved in ...
The seeds harvested at different times were dried to a constant weight at 80 °C and then ground using a ball mill. The oil was extracted from the embryos at 40, 54, 68, and 81 daa as previously described . The experiments were repeated in triplicate. RNA isolation, small RNA library construction, and sequencing
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Metabolic versatility of halotolerant and alkaliphilic strains of ...
Wheat straw black liquor is a notorious pulp mill wastewater with very high pH and pollution load. Two halotolerant and alkaliphilic bacteria, designated as Halomonas sp. 19-A and Y2, were isolated from wheat straw black liquor and shown to be able to use guaiacol, vanillin, dibenzo-p-dioxin, biphenyl and fluorene, as sole carbon and carbazole as sole carbon and nitrogen source at pH 9.5 and ...
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Effect of abscisic acid (ABA) and chitosan/nano-silica ... - ScienceDirect
The total RNA was extracted from the samples using a Column Plant RNAOUT Kit (Invitrogen, Beijing, China). 0.2 g of winter jujube peel powder from 40 fruits was used for measuring contents of total RNA extraction. The quality of the RNA solution was verified on an agarose gel and quantified using a Nanodrop 2000. Approximately 300 ng of RNA was ...
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Small RNA-Seq for Service Facilities - PerkinElmer Applied Genomics
An additional advantage CeGaT considered was the ability to do gel-free size selection during library preparation which is incorporated into the NEXTFLEX ® Small RNA-Seq Kit v3 protocol.. To offer their customers the best possible technical solution for small RNA sequencing, CeGaT works with the latest Illumina ® sequencing technologies including the Illumina ® NovaSeq ® sequencer.
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Co-circulation of Aedes flavivirus, Culex flavivirus, and Quang Binh ...
Then, 350 μl of supernatant from each ground sample was added to the processing cartridge and placed into to the MagNA Pure 96 System (Roche, Basel, Switzerland) for automated RNA extraction. The MagNA Pure 96 Cellular RNA Large Volume Kit, including DNase to obtain DNA-free RNA was used for the RNA extraction according to the manufacturer's ...
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Simultaneous Detection of Nine Grapevine Viruses by ... - Phytopathology®
Two plant total RNA extraction methods (silica capture and modified RNeasy method) and two RT-PCR systems (onestep and two-step) were evaluated to develop a reliable protocol for mRT-PCR. ... (Mill.) genetic resources by sequencing of barcode markers. 11 September 2015 | Journal of Genetics, Vol. 94, No. 3 ... 24 September 2012 | Journal of ...
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ancient origin of non-green phosphoribulokinase genes from two lineages ...
RNA extraction and cDNA library construction Cells of E. gymnastica, G. wittrockiana,andG. nostochi-nearum were crushed using ceramic beads and a Mixer Mill MM 300 (Qiagen, Hilden, Germany), and RNAs were subsequently extracted using the SV total RNA isolation system (Promega, Madison, WI, USA). Cells of
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Rapid Publication
RNA extraction and hybridization analysis. Immediately after dis-section, total RNA was extracted from testicular and renal tissue us-ing RNA Plus (Bioprobe Systems, Montreuil-sous-Bois, France) ac-cording to the manufacturer's instructions. Concentration of total RNA was estimated by spectrophotometry at 260 nm, considering
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Bead Mill Bacterial RNA Purification Kit - ELISABETH
The Bead Mill Bacterial RNA Purification Kit contains silica based spin-capture columns, pre-filled bead beating tubes and nontoxic reagents. The optimized reagents are designed specifically for RNA extraction from bacteria through bead mill homogenization and utilize highly denaturing buffer conditions to inactivate RNase's. After sample lysis ...
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Programmable low-cost DNA-based platform for viral RNA detection
Detection of RNA viruses in the clinical setting is typically performed using either immunological detection based on enzyme-linked immunosorbent assay (ELISA) to detect immunoglobulin M (IgM) antibodies or nucleic acid testing (NAT) based on a reverse transcription polymerase chain reaction (RT-PCR) assay to detect viral RNA ( 5 - 8 ).
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PureLink Plant RNA Reagent - Fisher Sci
1. ®Add 0.5 mL cold (4°C) PureLink Plant RNA Reagent to ≤0.1 gram of frozen, ground plant tissue. Mix by briefly vortexing or by flicking the bottom of the tube until the sample is thoroughly resuspended. 2. Incubate the tube for 5 minutes at room temperature. Note: Lay the tube down horizontally during incubation to maximize surface area. 3.
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Set screw homogenization of murine ocular tissue, including the whole eye
isolation of downstream analytes, such as protein or RNA. Additionally, automation of this step standardizes the time needed per sample, as well as negates differences in personnel training and technique, which, in turn, leads to accurate measurements and comparisons and increases reproducibility across experiments and laboratories.
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Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss ... - Hindawi
Received 14 Sep 2017. Revised 08 May 2018. Accepted 15 Jul 2018. ... For RNA extraction, eggs were thawed on ice and homogenized in TRIzol reagent (Thermo Fisher Scientific) using a manual and two homogenizer methods, Bullet Blender (bead mill) homogenizer (Next Advance) and TissueRuptor (Qiagen), in order to find the most efficient approach to ...
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Role of Anopheles Mosquitoes in Cache Valley Virus Lineage Displacement ...
Cache Valley virus (CVV; family Peribunyaviridae, genus Orthobunyavirus) belongs to the order Bunyavirales, which consists of 12 families and 46 genera that are major human, livestock, and plant pathogens ().CVV contains negative-sense, single-stranded RNA organized into 3 separate segments designated large (L), medium (M), and small (S) ().The L RNA segment encodes the RNA-dependent RNA ...
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So 7.1 Method for testing Tomato brown rugose fruit virus ... - NSHS
B. Option 2: IKA Mill (grinder) ... Add RNA extraction buffer to each ground subsample: 500 seed 1000 seed Tomato 6 ml 12 ml Pepper 12 ml -- ... ISHI-Veg, International Seed Federation. September 2019. Detection of infectious Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper seed. Version 1.3
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Optimizing Performance of SABC Comminution Circuit of the Wushan ... - MDPI
the processing plant operates with a standard sabc comminution circuit ( figure 1 ), and was the first to use the largest domestic φ8.8 × 4.8 m sag mill ('diameter' represents nominal diameter, and 'length' of the mill represents the effective grinding length inside the end liners and discharge grates), φ6.2 × 9.5 m overflow ball mill and closed .
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RNASep™ Prep - RNA Purification Column - ADS Biotec
RNASep ™ Prep is an RNA purification column that uses alkylated non-porous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres for high performance nucleic acid separations. These versatile columns can be used with a variety of HPLC systems for RNA analysis and purification. They are also ideal for the development of effective mRNA vaccines and other novel drug candidates.
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Two isoforms of the A subunit of the vacuolar H(+)-ATPase in ...
The plant vacuolar H(+)-translocating ATPase (V-ATPase, EC 3.6.1.34) generates a H+ electro-chemical gradient across the tonoplast membrane. We isolated two full-length cDNA clones (VHA-A1 and VHA-A2) from tomato (Lycopersicon esculentum Mill. cv. Large Cherry Red) coding for two isoforms of the V-ATPase catalytic subunit (V-ATPases A1 and A2).
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Dietary supplementation with olive mill wastewaters induces ...
RNA extraction. The total RNA from all 20 jejunum epithelial cell samples was isolated according to the Aurum Total RNA Fatty and Fibrous Tissue kit instructions (BioRad, CA, USA). ... Int J Mol Sci [Internet]. 2016 [cited 2017 Sep 12];17. Available from: ... Khoufi S, Aloui F, Sayadi S. Extraction of antioxidants from olive mill wastewater and ...
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Characterization of the first double-stranded RNA ... - Nature
Bacteriophage RNA isolation. g and passed through a 0.22-μm filter. The phages were further purified by PEG8000 precipitation. ... HPLC-MS data were processed by the Agilent Spectrum Mill ...
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Identification of candidate genes involved in the sugar metabolism and ...
The extraction of RNA, construction of cDNA libraries and the transcriptome sequencing assay were performed by Biomarker Biotechnology Corporation (Beijing, China). ... (Lycium chinense Mill.) . In this study, a total of 23.8 - 35.8 million clean reads were obtained from 9 cDNA libraries using this technology ... Published: 21 September 2017 ...
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Simple, rapid and reliable methods to obtain high quality RNA and ...
the methodology should satisfy the following practical issues: (i) the procedures should allow both rna and gdna to be isolated from the same disrupted material; (ii) the amount of starting material should permit the whole procedure to be conducted at microfuge scale, which will increase the number of samples that can be processed simultaneously, .
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RNA Salary in CA $73,468 (September 2022) ZipRecruiter
As of Sep 2, 2022, the average annual pay for the RNA jobs category in California is $73,468 a year. Just in case you need a simple salary calculator, that works out to be approximately $35.32 an hour. This is the equivalent of $1,412/week or $6,122/month. While ZipRecruiter is seeing salaries as high as $149,450 and as low as $23,833, the ...
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Matrix-bound nanovesicles within ECM bioscaffolds | Science Advances
Dermal ECM was then lyophilized and milled into particulate form using a Wiley Mill with a #40 mesh screen. Urinary bladder matrix ... MBV RNA was isolated using the SeraMir Kit (System Biosciences) according to the manufacturer's instructions. Before RNA isolation, MBV samples were treated with RNase A (10 μg/ml) (Applied Biosystems) at 37 ...
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How long can you keep the homogenised wheat grain tissue on dry ice ...
Evaluate the potential for extraction of RNA from S. cerevisiae cells on the Bead Ruptor 24 bead mill homogenizer. Theextraction efficiency and analyte integrity was evaluated. View
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Nucleic Acid Isolation - Sepmag
Jul 1, 2022Step 1: cell lysis. The first step in nucleic acid isolation is breaking apart the cell wall and/or membrane to release the genetic material. This is accomplished with the use of lysis buffer, rotor homogenizer, bead mill, freeze thaw cycles, or sonication. Lysis buffer contains a detergent to help break down cell membranes, and an enzyme such ...
WhatsApp:+8617329420102Genome-wide analysis of the bZIP gene family in Chinese jujube ...
Chinese jujube ( Ziziphus jujuba Mill.), a member of the Rhamnaceae family, is an important dry fruit and a traditional herbal medicine in Asia [ 24 ]. Both Chinese jujube and wild jujube, which are considered ideal fruit trees in arid and semiarid temperate regions, have strong tolerance to biotic and abiotic stress [ 25, 26 ].
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RNA-protein interaction mapping via MS2- or Cas13-based APEX ... - PNAS
Here, we present alternative methodologies for mapping RNA-protein interactions inside living cells, using both MS2 and CRISPR-Cas13 (17, 18) to target the proximity labeling enzyme APEX2 to specific RNAs.The rapid 1-min promiscuous biotinylation catalyzed by APEX enables proteomic identification (20-24) of endogenous interaction partners with much faster temporal resolution than BioID and ...
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Oil immersed lossless total analysis system for integrated RNA ... - Nature
Here the authors report OIL-TAS which integrates RNA extraction and detection into a single device. ... (CNC) three-axis mill (Tormach, PCNC 770). Following milling, the two pieces were thoroughly ...
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Successful tips of DNA extraction and PCR of plants for beginners - OAText
Add modified PVPP buffer and mix with leaf powder. Place at room temperature until the solution starts to thaw. Grind well again. Add samples to 15-mL or 50-mL plastic tubes, and heat in pre-heated heating block at 60°C for 30 min. Longer heating will cause DNA degradation. Heating at 80°C will slightly increase DNA yield.
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Rapid and Reliable Method of High-Quality RNA Extraction from Diverse ...
Received 19 August 2014; revised 19 September 2014; accepted 2 October 2014 ... Number of methods for RNA extraction in various plant species has been developed, most ... Ananas Mill., Clusia L., Euphorbia L.). Many protocols have been proposed for RNA extraction from specific type of tissue, such as the
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Intraspecific variation in defense against a generalist lepidopteran ...
frozen in liquid nitrogen and used for RNA isolation; samples harvested 48 h after damage by herbivores, MJ treatment, or wounding were also used for the analysis of defense metabolites. Glucosinolates extraction and HPLC analysis Extraction and analysis of GS were performed as described previously by Mucha-Pelzer et al. (2010) (Mucha-Pelzer et ...
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