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Although cones may contribute at the higher intensities, the prolonged, saturated responses are a hallmark of rod afterpotentials, indicating substantial rod input even above cone threshold. D: responses from 3 different cell types to 100-ms flashes of 200 R* · rod −1 · s −1 intensity are aligned to illustrate differences in kinetics and waveform.
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Plasma membranes of rod spherules and cone pedicles were labeled with anti-syntaxin 3 (dilution, 1:100) at 4°C overnight, followed by 2 h incubation with Cy3-conjugated donkey anti-rabbit IgG (3.7 μg/ml; Jackson ImmunoResearch) at room temperature. After washing in PBST, sections were mounted with Vectashield (Vector Laboratories).
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Extraction of total RNA and cDNA synthesis. Following light-dark cycle adaptation period (a minimum of 2 weeks for adults) guppies were euthanized in 1 g/L tricaine methanesulphonate, MS222, (Sigma ®) at 13:00 hours of the light phase.Sampling time was based on cichlid and zebrafish studies indicating that the highest level of cone opsin expression coincides with the end of the photopic day ...
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As described below in experiments with functional rod-less and cone-less mice (see Figs. 6, 7 ), we found that this approximate 8-log unit range of intensity encompasses both scotopic and photopic vision with a mesopic range separating the scotopic (<4.0 log cd m −2) and photopic (>2.0 log cd m −2) ranges. Mouse photopic vision is tuned to speed
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Rod and cone photoreceptors are coupled by gap junctions (GJs), relatively large channels able to mediate both electrical and molecular communication. Despite their critical location in our visual system and evidence that they are dynamically gated for dark/light adaptation, the full impact that rod-cone GJs can have on cone function is not known.
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The recording protocol incorporated rod-specific and standard bright-flash ERGs, both of which were recorded after a minimum dark adaptation time of 20 min. Photopic 30-Hz flicker stimulus and transient photopic cone ERGs were recorded after 10 min of light adaptation [ 24 ]. Genetic analysis
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Despite important differences between cone and rod photoreceptors (rods), e.g. with respect to the glutamate release ... 2013b), but were proposed for other non-primate mammals such as rabbits (Mills et al. 2014). In addition, ... All authors have approved the final version of the manuscript and agree to be accountable for all aspects of the ...
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Cells and layers of the retina (coronal view) Rods. Rods are cylindrical shaped photoreceptors.They are more numerous than cone cells, with an estimated 92 million rod cells located in the human retina.They function best in low intensity light (scotopic) and are thus responsible for vision in dimly lit surroundings, such as at dusk. Rod outer segments are cylindrical in shape, consisting of ...
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The cone cells appeared as bright spots that were 2-5 μ m in diameter, and the examiners manually selected areas (50 × 50 pixels), while excluding the areas where in retinal vessels or artifacts...
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